Deconvolving the transcriptional signature of sexual commitment in Plasmodium falciparum

Single Cell RNAseq dataset: For these experiments we used two culture- adapted P. falciparum strains: Pf2004 from West Africa and Dd2 from Southeast Asia33-35. At 26-28 hours post invasion (hpi), synchronised parasite cultures were subjected to either mFA medium or serum-supplemented control medium. After 16 and 22 hours of further incubation (due to observed differences in asexual blood stage cycle length between the two strains), respectively, two time points were collected for analysis and samples from both strains were pooled per condition (mFA or serum samples). Single cell transcriptomes were captured using the 10X Genomics droplet-based Chromium platform.