Expression data from rosettes and roots of Arabidopsis Col-0 and rpt2 grown under sulfur deficiency.

Bearing in mind the prevalent occurrence of sulfur deficiency in soils, it is highly essential to comprehend the molecular processes of plant response to the changing conditions of sulfur nutrition. As there is an increasing understanding of ubiquitin-proteasomal protein degradation system participation in nutrient deficiency response, we could predict its input to the sulfur metabolism as well. Therefore, we decided to investigate the consequences of proteasome malfunction in Arabidopsis in sulfur deficient conditions. This study presents the transcriptomic response profiles of sulfur-deficient rosettes and roots of Arabidopsis thaliana mutant plants with proteasomal malfunction. Arabidopsis thaliana plants (Col-0 and rpt2-3a mutant) were grown hydroponically under short-day conditions (8h light / 16h dark cycles) under full nutrient supply for 5 weeks. After that the plants were either grown as previously or transferred to sulfur deficient nutrients for 5 days. Arabidopsis rosette and root material was harvested separately and used for RNA extraction and hybridization on Affymetrix microarrays. Three biological replicates from each condition were analyzed.