Transcriptome-wide analysis of the adult mouse heart

Here we present 3D-cardiomics, a three-dimensional gene expression atlas of the murine adult heart generated from RNA-sequencing of 18 anatomical sections. Analysis of this dataset revealed regional synexpression groups, including known cardiac-markers and novel compartment-specific genes. In addition, we present a novel visualization interface that facilitates interactive gene expression navigation, synexpression analysis and differential gene expression across sections. Our study provides a unique framework to explore gene expression in an adult mouse heart where information is scarce, and enables the identification of spatially-restricted genes at an unprecedented resolution.