Effect of FL-Fc-DM1 drug on gene expression of Molm-13 cells

To eliminate cell-cycle arrested AML cells, we developed a FL-Fc-DM1 drug by conjugating FLT3 liangd Fc fusion protein and mertansine. We used the Molm-13 cells as a AML model. The total mRNA of Molm-13 cells treated with different concentration of FL-Fc-DM1 were sequenced. Molm-13 cells were cultured in RPMI 1640 medium supplemented with 10% FBS. In the control group, Molm-13 cells were cultured for 1 day. In the FL-Fc-DM1 treatment groups, Molm-13 cells were treated with FL-Fc-DM1 (1 and 3 nM) for 24 hours. We than analyze the transcriptome differences between different groups.