Directional RNA sequencing of mRNA from Sinorhizobium meliloti cells grown with limiting phosphate and with excess phosphate.. Sinorhizobium meliloti

Analysis of the RNA-seq reads confirmed that transcription of many of the previously identified Pho-regulon genes of Sinorhizobium meliloti was regulated by the availability of inorganic phosphate in the media. The transcriptional start sites of many of the Pho-regulon genes as determined by RNA-seq reads were found to correspond to those determined from primer extension analysis. Overall design: mRNA from S. meliloti RmP110 grown in MOPS + 2 mM Pi and MOPS + 20 µM Pi – directional RNA-seq via Illumina sequencing