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IPSC-derived neural precursor cells differentiation and characterization.

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https://figshare.com/articles/dataset/_IPSC_derived_neural_precursor_cells_differentiation_and_characterization_/1607776
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(A) Protocol for direct differentiation of human stem cell lines (IPSC) into neural precursor cells. After EBs formation from day 4–9, cells were differentiated as embryoid bodies from day 9–16 in neural induction media where neural rosette structure was selected and plated and second passage cells were analyzed. (B-D) Representative immunostaining results for NPC cultures from different IPSC (Control, AMN and cALD) shows SOX9+, PAX6+ and Nestin+ NPC cells (scale bars, 200 μm). (E) Summary chart of observed positive and negative markers for NPC characterization.
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2016-02-23
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