Altered distribution of erythroid progenitors in ISG15-/- bone marrow and splenic cells.

(A) Blood was collected from mice at 8–10 weeks of age. Hematologic measurements were performed on a MS9 Hematology Analyzer (MELET SCHLOESING Laboratoires). The data are mean +/- SEM (N = 14). RBC indicates red blood cells; HGB, hemoglobin; MCH, mean corpuscular hemoglobin; MCHC, MCH concentration (calculated); HCT, hematocrit; MCV, mean corpuscular volume; RDW, RBC distribution width, Retic, reticulocytes; PLT, platelets; WBC, white blood cells; MONO, monocytes, LYMP, lymphocytes and GR, granuloctes. (B) Quantitative analysis of the distribution of the different erythroblasts subsets in age-matched WT versus ISG15-/- mice. Flow cytometry analyses using the cell surface markers CD71 and Ter119 of bone marrow and spleen cells isolated from WT or ISG15-/- mice (as described in Figure 1A). Dead cells (7AAD+) were excluded from the analysis. The data are mean +/- SEM (n = 13). (C) 2.105 BM and 2.106 spleen cells from mice at 8–10 weeks of age were used to assay BFU-E and CFU-E numbers in MethoCult M3334 (StemCell Technologies). For CFU-E assays, colonies were counted at day 2 and for BFU-E assay, at day 4. The data are mean +/- SEM (n = 11).