Projects related to development of new methods, the reduction per study and estimated annual reduction.

NA = Not analyseda) Not possible to estimate as the test/study is performed when requested and this varies substantially between years.b) Not possible to estimate the effects on reduction of animal use or replacement of animal studies today.c) Not possible to estimate as the number of biomarker studies and study design varies depending on present drug projects and safety assessment issues. Probably a reduction of more than one hundred animals per year. To be evaluated after 2–3 years.d) Possible risk for interference with observations of clinical signs is addressed and during scientifically investigation and evaluation before implementing the method as default.