Gene expression during embryonic wound healing of Xenopus laevis after inhibition of production of NO.

We analyzed the gene expression profile during the first 90 minutes of embryonic wound healing and compared it with embryos with a chronic problem with the production of nitric oxide (NO) (embryos originating from oocytes that were injected with morpholinos against nos1 and nos3) and embryos with an acute problem with the production of NO (embryos incubated in a solution containing TRIM). Overall design: Embryos at stages 24-26 were injured using forceps (at middle ventral side) and five pieces of the wounded tissue were collected from each replicate.