Genetic and Epigenetic Determinants Establish a Continuum of Hsf1 Occupancy and Activity Across the Yeast Genome

We performed ChIP-seq of Hsf1 under non heat shock, 5-minute heat shock and 120 minute heat shock conditions. We used the conditional chemical genetics approach known as “anchor away” (AA) to rapidly inactivate Hsf1. We coupled Hsf1-AA to and nascent RNA seq (NAC)-seq to define the genes whose expression depends on Hsf1 during heat shock.本研究在非热激、5分钟热激及120分钟热激三类条件下，对热休克因子1（Hsf1，Heat Shock Factor 1）开展了染色质免疫共沉淀测序（ChIP-seq，Chromatin Immunoprecipitation sequencing）。我们采用了名为"锚定撤离（Anchor Away，AA）"的条件性化学遗传学方法，以快速失活Hsf1。我们将Hsf1-AA系统与新生RNA测序（NAC-seq，Nascent RNA Sequencing）相结合，以此明确热激过程中表达依赖于Hsf1的基因。