Revealing the virome of Ixodes ricinus ticks from northern Europe. Ixodes ricinus

RNA/transcriptome sequencing was performed on six libraries consisting of 33 Ixodes ricinus nymphs and adults sampled from six localities in Norway. For all libraries, ribosomal RNA (rRNA) was removed using the Ribo-Zero Gold (epidemiology) Kit (Illumina) following the manufacturer’s instructions. Libraries were constructed for all rRNA-depleted RNA-samples using the KAPA Stranded RNA-Seq Kit following the manufacturer’s instructions. Sequencing libraries were pooled in equimolar amounts. All libraries were sequenced on a single lane (paired-end, 125bp read-length) on an Illumina HiSeq 2500 platform