seq-RNA analysis of PBMC in stroke patients with severe atherosclerosis and healthy donors

The goals of this study are to evaluate the gene expression of PBMC in stroke patients with atherosclerosis compared with healthy donors. PBMC mRNA profiles of stroke patients and healthy donors were generated by sequencing using Illumina. We detected 60613 transcripts, and there are there are 470 significant genes expression upregulated (fold change > 2 and p value < 0.05) and 355 downregulated (fold change < 0.5 and p value < 0.05) in atherosclerotic patients versus healthy individuals. We found that differentially expressed genes were significantly enriched among immune response and virus defense categories by functional KEGG analysis, including TNF signaling pathway, Toll-like receptor signaling pathway, NF-kappa B signaling pathway, NOD-like receptor signaling pathway, RIG-like receptor signaling pathway and apoptosis. Overall design: PBMC mRNA profiles of stroke patients with severe atherosclerosis (n=4) and healthy donors (n=3)