unigene.fasta.SSR_primer.xls
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https://figshare.com/articles/dataset/unigene_fasta_SSR_primer_xls/5383648
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资源简介：
The
searching principle was that only di-, tri-, tetra-, penta- and
hexa-nucleotides with a minimum of 8, 5, 5,4 and 3 repeats will be considered
as SSR loci. The primers of each EST-SSRs were designed using Primer Premier
5.0 (Premier Biosoft International, CA, USA) with
following criterias: PCR fragment size- 100-350 bp; primer length- 16-24 bp;
Tm- 55-65 °C; GC content- 40-60 %.
创建时间：
2017-09-07
