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PAM specificities of Cas9 variants in endogenous loci
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创建时间：
2018-11-30
相关数据集
Use of CRISPR/Cas9 with homology-directed repair to silence the human Topoisomerase IIα intron-19 5' splice site: Generation of etoposide resistance in human leukemia K562 cells. Use of CRISPR/Cas9 with homology-directed repair to silence the human Topoisomerase IIα intron-19 5' splice site: Generation of etoposide resistance in human leukemia K562 cells
Results support the important role of both TOP2α/170 and TOP2α/90 as determinants of sensitivity/resistance to TOP2α-targeting agents. Overall design: CRISPR/Cas9 with homology-directed repair was uti
NIAID Data Ecosystem40
Linking CRISPR/Cas9 double-strand break profiles to gene editing precision with BreakTag [hiplex2]. Linking CRISPR/Cas9 double-strand break profiles to gene editing precision with BreakTag [hiplex2]
Here we developed BreakTag, a versatile, highly parallel and scissile-aware methodology for the profiling of Cas9-induced DNA double strand break (DSBs), to identify molecular determinants influencing
NIAID Data Ecosystem10
Primer sequences for sgRNAs, PCR sequencing, and qRT-PCR.
sgRNAs: OCT4 exon 2 and exon 5 targeting sgRNA primer; PCR sequencing: Target site mutation check primer; qRT-PCR: mRNA expression check primer. (PDF)
Figshare2015-12-03 更新00
File S2.zip
Annotated sequence files in .ape format (comparable with Genbank) of knock-ins generated by CRISPR/Cas9-mediated genome editing. Unmodified genomic sequence is also provided.
Figshare2023-03-14 更新20
EGAS00001006170-sc-2023-10-20T08:25:08Z - samples
8 cell pellet samples for genomic DNA extraction. CRISPR PCR1 and PCR2 indexing - Please use standard Kosuke primers.EGA dataset EGAD00001012233
NIAID Data Ecosystem00
