Two-photon fluorescence microscopy image stacks of human brain sections (grey and white matter)

Two-photon fluorescence microscopy (TPFM) image stacks of human brain sections including grey matter (Ng=10) and white matter (Nw=10), considered in the validation of the 3D fiber orientation analysis pipeline proposed in: "Fiber enhancement and 3D orientation analysis in label-free two-photon fluorescence microscopy". Human brain tissue was preliminarily treated for TPFM following the label-free MAGIC preparation technique, presented in (Costantini et al., Scientific Reports 2021). The PSF of the TPFM system has a FWHM of (0.692, 0.692, 2.612) μm along the x, y, and z axes, respectively, whereas the adopted voxel size is 0.88 μm x 0.88 μm x 1 μm.