Locus-specific and activity-independent gene repositioning during early tumorigenesis

The nucleolus during mammary epithelial differentiation and early mammary tumorigenesis. (A) MCF-10.B2 cells grown for 20 days under 3D growth conditions with ten days of constitutive activation of ErbB2 (Day 20, ErbB2; bottom left panel) were stained with ANA-N antibody to detect nucleoli (green) and counterstained with DAPI to delineated nuclei (blue). The stacks shown is for the projected image (for only part of this stack, ~5um)shown in the manuscript.