T Cell Subset and TCR Repertoire Analysis in Esophageal Squamous Cell Carcinoma

Tumor immune response is dependent on the T cell subset and T cell receptor (TCR) repertoire. We aimed to identify the T lymphocyte subsets and the shared TCR repertoire that comprised in esophageal cancer. Fifty-three cases were enrolled, and immunohistochemical analysis of CD3, CD8, CD45RO, FOXP3, PD-L1, HLA class I and AE1/AE3 was performed. The clinicopathological significance of *immuno relation high (IR-Hi)* was assessed, and further stratification was performed according to the expression of markers of different T cell subsets. Adaptor ligation PCR and next-generation sequencing were performed to explore the TCR repertoire highly shared in the *IR-Hi* group.