List of FLC-evolved strains with different ERG251 point mutations.

ERG251 mutations were identified in three independent FLC evolution experiments within two distinct genetic backgrounds (SC5314-derived Sn152 and BWP17, and P75063). Multiple single-colony strains from the same evolved population are organized into the same row. ERG251 point mutations are annotated on the mutated allele, and represented as allele A/allele B, except for AMS5615 where the chromosome region containing ERG251 became homozygous prior to acquisition of the point mutation. ERG251 in the P75063 background is homozygous.