Oxygen consumption (pmol O2 × mg-1 × s-1) of kidney mitochondria from Sham, CL and UUO mice.

Assays were carried out in the presence of rotenone and after addition of succinate − substrate for complex II − under phosphorylating and non-phosphorylating conditions (states 3 and 4 are with and without ADP, respectively), with ATP synthesis blocked (oligomycin), and in the uncoupled state of respiration (FCCP). Uncoupled respiration was measured after adding 1.5 μM FCCP.