Sequencing of RNA Isolated Through RNA Immunoprecipitation (RIP) of Murine Cortical Tissue Using STAU2 Antibody

We report here the sequencing and analysis of RNA isolated through RNA immunoprecipitation (RIP) of embryonic mouse cortical tissue. STAU2 antibodies were used to perform these RIPs at four different ages, and the RNA was compared against matching input samples. This allowed characterization of putative STAU2 binding sites in different RNA classes over the course of development, important as STAU2 is a known double-stranded RNA binding protein with a role in asymmetric division of neural progenitor cells. Examination of STAU2-bound RNA at four discrete time points during murine cortical development. Three STAU2 RIP samples (biological replicates) and three input samples (biological replicates) were analyzed at each time point.