fecal microbiota (16S) of WT or endothelial cell specific human IGF1R overexpressing mice hIGFREO fed high fat diet with and without antibiotic cocktail treatment. Mus musculus

hIGFREO mice with endothelial cell-specific overexpression of the IGF-1 receptor (previously described Imrie et al, 2012) and their wild-type control littermates (WT) mice are placed on high fat diet (60% of energy from fat- F1850, Bioserve) at 8 weeks of age for 8 weeks. At 16 weeks (8 weeks post diet), mice are randomized into control or antibiotic cocktail (ampicillin (1/gl), metronidazole (1/gl), neomycin trisulfate (1/gl) and vancomycin (0.5/gl) administered through the drinking water) for 8 additional weeks, while maintained on the high fat diet.