Behavioral profile of PTZ-induced seizures in adult zebrafish.

The main characteristic seizure behavior induced by 5–15 mM PTZ and DZP/10 mM PTZ treatments during 20 min (n = 12). (A) Seizure score (only the highest score reached was consider in each interval) and cumulative frequency (B). Data are represented as median ± interquartile range and as the animal index (%) that reached the scores across time, respectively. (C) Seizure intensity during distinct moment tests (0–150, 150–300, and 300–1200 s) evaluated by the area under curve observed for each treatment. (D) Latency to score 4 onset. Data from seizure intensity and latency are represented as mean ± S.E.M and analyzed by one-way ANOVA followed by Bonferroni’s test as post-hoc. Distinct letters indicate statistical differences among PTZ-treated groups (gray bars). The DZP/10 mM PTZ is represented as black bars and compared to 10 mM PTZ group by the Student’s t test. *indicates significant difference between groups.