Effect of the TBSV-encoded P19 RNA silencing suppressors on the expression of the GUS reporter transgene in transgenic sugarcane.

Average GUS activity was measured in leaves of 4-month-old sugarcane transgenic lines co-expressing pUbi:GUS and pUbi:P19 (7 lines; 41 plants analyzed). pUbi:GUS transgenic (3 lines; 5 plants analyzed) and non-transgenic sugarcane (3 plants) were included as controls. For each set of experiments, the range of GUS activity values is indicated in parenthesis. Values represent three biological samples and three technical repeats, and are reported with the standard error. Values with the same letter are not significantly different (p>0.05). 20% and 39% represent the percentage of plants that are transgenic for the P19 and GUS genes.