Human prostate cancer bone metastases have an actionable immunosuppressive microenvironment

We collected unique sets of tissue fractions from each patient: solid metastatic tissue (Tumor fraction), liquid bone marrow at the site of the metastasis (Involved), as well as liquid bone marrow from a vertebral body distant from the tumor site (Distal). This allowed for a comparison within the same individual, controlling for inter-individual variation. Bone marrow samples from patients undergoing hip replacement surgery (Benign) served as a non-malignant comparator group. The transcriptional composition of all samples was assessed using single-cell RNA-seq (scRNA-seq). Bone marrow were disaggregated and profiled by 10X Genomics Single cell 3 gene expression V2. **The records have been updated with raw data for samples GSM4274678-GSM4274709 on May 12, 2022.

我们从每位患者处采集了专属的组织分群数据集：实体转移组织（肿瘤组分，Tumor fraction）、转移部位液态骨髓（受累骨髓，Involved）以及远离肿瘤部位的椎体来源液态骨髓（远端骨髓，Distal）。该设计可实现同一患者体内的组间比对，从而控制个体间变异带来的混杂影响。我们选取接受髋关节置换手术患者的骨髓样本（良性对照，Benign）作为非恶性对照组别。所有样本的转录组特征均通过单细胞RNA测序（single-cell RNA-seq，scRNA-seq）进行分析。骨髓样本经解离后，采用10X Genomics单细胞3'基因表达V2试剂盒完成表达谱检测。**本数据集已于2022年5月12日更新了样本GSM4274678至GSM4274709的原始数据。**