File S1 - Disruption of the Endothelial Barrier by Proteases from the Bacterial Pathogen Pseudomonas aeruginosa: Implication of Matrilysis and Receptor Cleavage

This file contains Protocol S1 and Figure S1 through Figure S4. Protocol S1, Preparation of extracellular bacterial secretomes and proteinase activity assays. Figure S1, Profile of proteinases in the secretome of wild type and LasB-deficient strains of P. aeruginosa. Figure S2, Clinical pseudomonal secretomes drastically alter adherence of human endothelial cells in culture in a LasB-dependent fashion. Figure S3, Residual adherent endothelial cells in the presence of pseudomonal secretomes are fully viable. Figure S4, The LasB-containing secretome does not proteolytically alter the major endothelial integrins. (DOC)