Combined single-cell and spatial transcriptomics reveal the precise cellular states of postnatal cerebellar granule cells and connection with medulloblastoma

The progenitor/differentiation states of cerebellum granule cells have been investigated for many years. Deregulation of granule cells development is associated with the pathogenesis of medulloblastoma (MB).Our study reveals the cellular and spatial organization of cerebellar granule cells and provide direct evidence showing the similarity and discrepancy between normal development and tumorigenesis. Single cell mRNA profiles of 2 samples from Math1-GFP mice, 2 samples from Dcx-DsRed mice, 2 samples from WT mice and 3 SHH MBs from Patched+/- mice were generated by 10X Genomics plartform, without technical replication, and sequenced by NovaSeq 6000 (Illumina). Spatial transcriptomics (ST) profiles of 2 samples of P7 WT mice were generated by 10X Genomics plartform, without technical replication, and sequenced by NovaSeq 6000 (Illumina).