Analysis of Pyrimidine metabolism in cold treated Arabidopsis thaliana plants by transcriptome study. Pyricold

We analyzed a knockdown mutant für ATCase (aspartate transcarbamoylase, atc#1) the gene product catalyzed the first committed step in Pyrimidine de novo synthesis . Control plants (Col-0) and atc#1 were grown for six weeks and then trnsferred to cold for one or three days f cold (4°C). Subsequently overground material was harvested, RNA isolated and used for next generation sequencing to (transcriptome analysis). Each genotype and treatment was done with three biological replicates