DNA methylation (RRBS) profiling of the mouse myoblasts cell line C2C12 cultured in four temperature-treatment conditions

This experiment aims at investigating the effects of heat-stress on DNA methylation changes of the muscle cells in in-vitro model using the mouse myoblast cell line C2C12. Four incubation temperatures were set as a treatment condition at 35°C (cold stress), 37°C (control), 39°C (mild heat-stress) and 41°C (severe heat-stress). The cells were cultured under the treatment conditions for 72 hours prior to harvesting the cells for DNA extraction. The experiment was done in three independent replications. RNA-seq of matched samples has been profiled (E-MATAB-9895).