Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Cd226-/- BMDM Transcriptomes

Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare WT-BMDM vs. cd226-BMDM transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis Overall design: BMDM mRNA profiles of 56-day old wild type (WT) and Cd226-/- mice