Genome-wide maps of UV damage repair by yeast CPD photolyase

Here, we describe a genome-wide map of photoreactivation repair of UV-induced cyclobutane pyrimidine dimers (CPDs) in yeast (Saccharomyces cerevisiae) by the endogenous yeast CPD photolyase. CPDs were mapped at single nucleotide resolution across the yeast genome using the CPD-seq method immediately after UV radiation (0min) and following 30 or 60min photoreactivation with UVA light. Photoreactivation repair by photolyase was mapped in both wild-type yeast and rad14? cells deficient in nucleotide excision repair (NER). We used these data to analyze CPD repair by photolyase in yeast genes, nucleosomes, and transcription factor binding sites. Overall design: We used the CPD-seq method to map UV-induced cyclobutane pyrimidine dimers (CPDs) at single nucleotide resolution across the yeast genome, both immediately after UV-radiation of yeast cells (UV 0min) and following 30min or 60min of photoreactivation with UVA light. CPD-seq was used to analyze photolyase repair in both wild-type (WT) and NER-deficient (rad14?) yeast cells.