Multiple RNA structures affect translation initiation and UGA redefinition efficiency during synthesis of selenoprotein p

Mice carrying genomic deletions of SECIS1 or SECIS2 in the Selenop gene were euthanized and liver tissue was harvested and processed for RNA-Seq and ribosome profiling. From these studies, we determined the affect of SECIS1 and SECIS2 on UGA redifinition across the Selenop gene. Overall design: Gene expression analysis of liver tissue from mice carrying genomic deletions of the 3'' UTR SECIS1 or SECIS2 elements of Selenop