Microglia regulate GABAergic neurogenesis in prenatal human brain through IGF1 [MGEorganoid_scRNAseq]

We conducted scRNAseq to investigate the transcriptomics of induced microglia (iMG) and iMG containing MGE-oriented organoids. Overall design: Human embryonic stem cells-induced microglia (iMG) were transplanted to 4-week-old MGE organoids. We conducted scRNAseq to investigate the transcriptomics of 6-week-old MGE organoids with and without iMG. We also used Fluorescence-activated cell sorting (FACS) to enrich GFP-labelled iMG and condcuted scRNAseq.