Ecological effects of soil microbiota and tobacco system induced by pesticides nanomaterials and the two compound stress

As a widely cultivated crop, the stability of the tobacco (Nicotiana tabacum) ecosystem is of irreplaceable significance for maintaining the global ecological balance. However, soil pollutants generated by human production activities are continuously posing a potential threat to the tobacco field ecosystem. Therefore, in this study, tobacco was selected as the research object. By simulating field pollution, 16S rRNA amplicon sequencing and tobacco growth index determination was carried out to explore the ecological effects of tobacco rhizosphere soil microorganisms and tobacco on nanomaterial silica (SiO2), chemical pesticide triflumezopyrim (TFM) and their combined use (SiO2@TFM). It was found that the addition of different pollutants significantly reduced the species richness of soil microorganisms and changed their community composition. In terms of affecting tobacco growth and development, SiO2 and TFM treatments increased the growth rate of tobacco stem circumference and effective leaf number. In summary, different pollutants will have a complex impact on the ecological network between tobacco rhizosphere microorganisms and tobacco, and poses a potential threat to the structural stability and functional integrity of the tobacco field ecosystem.