Characterization of the novel spontaneously immortalized rat Müller cell line SIRMu-1

Müller cells (MCs) play a crucial role in the retina, and cultured MC lines are an important tool with which to study MC function. Transformed MC lines have been widely used; however, the transformation process can also lead to unwanted changes compared to the primary cells from which they were derived. A monoclonal spontaneously immortalized rat Müller cell line, SIRMu-1, was derived from primary rat MCs and characterized by RNA-sequencing (in addition to immunofluorescence and western blotting) in comparison to primary MCs and the SV40-immortalized MC line, rMC-1.米勒胶质细胞（Müller cells, MCs）在视网膜中发挥关键作用，体外培养的MC系是研究MC功能的重要工具。转化型MC系已得到广泛应用，但相较于其来源的原代细胞，转化过程往往会引发非预期的细胞特性改变。单克隆自发永生化大鼠米勒胶质细胞系SIRMu-1源自大鼠原代MCs，研究人员通过RNA测序（RNA-sequencing）（辅以免疫荧光（immunofluorescence）与蛋白质印迹（western blotting）技术）对其进行表征，并将其与原代MCs以及SV40永生化MC系rMC-1开展对比分析。