Genome Mining dataset of Stenotrophomonas hibiscicola ShG5i Isolated from Wheat (Triticum aestivum cv. GR119) Seed

This dataset contains genome mining and functional annotation results of the seed endophytic bacterium Stenotrophomonas hibiscicola strain ShG5i, isolated from wheat (Triticum aestivum cv. GR119) seed collected from the IBGE, GAU, Gazipur, Bangladesh (24.0360° N, 90.3963° E), and kindly provided by Prof. Yukio Tosa (Kobe University, Japan). The complete genome was generated using Oxford Nanopore sequencing and assembled into a single circular chromosome of approximately 4.38 Mb with a GC content of 66.5%. The genome was annotated and analyzed to explore biosynthetic potential and plant growth-promoting traits. Genome assemblies and associated sequencing data are publicly available under the following accession numbers: Feature ShG5i GenBank JBTONN000000000 Assembly GCA_054552555.1 BioSample SAMN54560989 SRA SRS28243357 16S rRNA PZ230247 Genome mining using PGPg_finder v1.1.0, antiSMASH v8.0.4, and the RASTtk annotation pipeline (RAST v2.0 server) identified a total of 22 biosynthetic gene clusters (BGCs). Detected clusters include non-ribosomal peptide synthetases (NRPS), ribosomally synthesized and post-translationally modified peptides (RiPPs), terpenes, arylpolyenes, lanthipeptides, and hybrid clusters. A high-confidence NRPS-based metallophore biosynthetic gene cluster showed similarity to the 2,3-dihydroxybenzoylserine pathway, suggesting a role in iron acquisition. Additional clusters, including arylpolyene and RiPP-like systems, indicate potential functions in oxidative stress protection and microbial interactions. PGPg_finder analysis identified genes associated with plant growth-promoting traits, including iron acquisition systems (tonB, feoAB, afuA/fbpA), phosphate solubilization (phoD, phoN, pstABC, phnD), potassium uptake (kdp system), and sulfur metabolism (cys and ssu genes). Genes related to phytohormone-associated metabolites (speA, speD, speE, budB) and vitamin biosynthesis pathways (thi, pdx, fol) were also detected. Additionally, the presence of phenazine biosynthesis genes (phzF, yddE) and chitin-binding protein genes suggests a potential role in plant protection and antagonistic activity against phytopathogens. Overall, the genome features and biosynthetic gene cluster profile of Stenotrophomonas hibiscicola ShG5i highlight its metabolic versatility and potential role as a plant-associated beneficial microbe with applications in sustainable agriculture and biocontrol.