Summary of Ae. aegypti and P. xylostella injections for Cre and FLP injections.

Ae. aegypti (‘Aae’) strains were injected with Cre and FLP in parallel for direct comparison. The number of Ae. aegypti pools with progeny apparently lacking the AmCyan marker (due to weak fluorescence) is shown (‘marker loss’); this was not a problem with the P. xylostella (‘Pxy’) strain so values in this column reflect true marker loss. Excision events (E) were confirmed by PCR and sequencing, and the minimum calculated excision frequencies (Efficiency) are shown as a percentage.Summary of Ae. aegypti and P. xylostella injections for Cre and FLP injections.