Data and code from: Ultra-wide-field, deep, adaptive two-photon microscopy for multi-scale neuronal imaging

Observing the activity patterns of large neural populations throughout the
brain is essential for understanding brain function. However, capturing
neural interactions across widely distributed brain regions from both
superficial and deep cortical layers remains challenging with existing
microscopy technologies. Here, we introduce a state-of-the-art two-photon
microscopy system, ULTRA, capable of single-cell resolution imaging across
an ultra-large field of view (FOV) exceeding 50 mm2, enabling deep and
wide field in vivo imaging. To demonstrate its capabilities, we conducted
a series of experiments under multiple imaging conditions, successfully
visualizing brain structures and neuronal activities spanning a spatial
range of over 7 mm from superficial layers to depths of up to 900 μm,
while covering a volume of 45.24 mm3 in the mouse brain. This versatile
imaging platform overcomes traditional spatial constraints, providing a
powerful tool for comprehensive exploration of neuronal circuitry over
extensive spatial scales with cellular resolution. This dataset contains
data sufficient to reconstruct certain of the figures in the paper.