Hypoxia and the metabolic phenotype of Daphnia

Environmental hypoxia typically induces phenotypic remodelling in animals, including bolstered oxygen transport and storage, and glycolytic enzyme activities, typically coordinated by the transcription factor hypoxia-inducible factor (HIF). The calanoid copepod Daphnia responds to hypoxia by a HIF-dependent increase in expression of extracellular hemoglobin (Hb), however little is known about the glycolytic response. We compared the Daphnia from 4 lakes in terms of transcriptome using RNASeq analysis of red versus pale animals. Cluster analysis showed that the impact of lake was much greater than the impact of colour. However, in each lake there were a series of colour dependent differences, including many of the known targets of HIF in mammals. Overall design: Two phenotypes each for 4 lakes (eight groups)