Single nucleus RNA-seq of Kdr-FACS cells from late embryonic mouse kidney

Here, we performed single nuclear RNA sequencing (snucRNA-Seq) to interrogate embryonic renal endothelial cells. We identified nine different endothelial cell types, and validated expression of novel candidates using multiplex RNAScope. BBC2 SCLL cells were transplanted into wild type Balb/C micen. Peripheral blood samples were collected from naive mice and leukemic mice at day 11 with intermediate stage and day 14 with late stage disease. The leukocytes were processed for scRNA-Seq libraries using the Chromium Controller (10X Genomics) and the Chromium 3 Single Cell mRNA-seq V3 reagents. The scRNA-Seq libraries were sequenced using an Illumina NovaSeq 6000 System.本研究采用单细胞核RNA测序（single nuclear RNA sequencing，snucRNA-Seq）对胚胎肾内皮细胞展开研究。我们共鉴定出9种不同的内皮细胞亚型，并利用多重RNAScope（multiplex RNAScope）验证了新型候选靶点的表达特征。将BBC2 SCLL细胞移植至野生型Balb/C小鼠体内，随后分别采集正常未处理小鼠、疾病中期（造模后第11天）的白血病小鼠以及疾病晚期（造模后第14天）的白血病小鼠的外周血样本。采用10X Genomics公司的Chromium控制器（Chromium Controller）及Chromium 3'单细胞mRNA测序V3试剂对分离得到的白细胞进行单细胞RNA测序（scRNA-Seq）文库构建，最终使用Illumina NovaSeq 6000测序系统完成文库测序。