SNP markers used for QTL mapping in the inbred lines

Young leaves of the 175 inbred lines and their seven parents were collected from seedlings grown in a greenhouse. About 200 mg bulk leaf sample from three plants of a line was placed in 2 ml safe-lock Eppendorf tube and stored at ‒80 ˚C for one night prior to crushing using a Mixer Mill (TissueLyser II, Qiagen, Germany). Genomic DNA was extracted using SIGMA DNA extraction kit (Sigma-Aldrich, St. Louis, MO, USA) following the manufacturer’s instruction. DNA concentration and purity of the samples were assessed using a NanoDrop 2000c spectrophotometer (Thermo Scientific, Wilmington, DE, USA). The samples were processed and sequenced using tunable genotyping-by-sequencing (tGBS®) method by Data2Bio (Ames, IW, USA). Genomic DNA was digested using two restriction enzymes NSpI (5′-RCATG^Y-3′) and BfuCI/Sau3AI (5′-^GATC-3′) which created 3´and 5´overhangs, respectively. Two single-stranded oligos, one containing a sample-specific internal barcode and the other a universal oligo, were lig..., , , # SNP markers used for QTL mapping in the inbred lines

Dataset DOI: [10.5061/dryad.8sf7m0crw](10.5061/dryad.8sf7m0crw)

## Description of the data and file structure

The dataset consists of a Brassica_napus_SNP-SNP5743_6.csv

SNP discovery using genotype by sequencing

Young leaves of the 184 inbred lines and their seven parents were collected from seedlings grown in a greenhouse. About 200 mg bulk leaf sample from three plants of a line was placed in a 2 ml safe-lock Eppendorf tube and stored at 80 °C for one night prior to crushing using a Mixer Mill (TissueLyser II, Qiagen, Germany). Genomic DNA was extracted using the SIGMA DNA extraction kit (Sigma-Aldrich, St. Louis, MO, USA) following manufacture’s instruction. DNA concentration and purity of the samples was assessed using a NanoDrop 2000c spectrophotometer (Thermo Scientific, Wilmington, DE, USA). The samples were processed and sequenced using tunable genotyping-by-sequencing (tGBS®) method by Data2Bio (Ames, IW, USA). Genomic...,