RNAseq analysis of retinal microglia during early diabetes
收藏NIAID Data Ecosystem2026-04-30 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP226697
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资源简介：
We report the use of RNAseq to identify gene changes in isolated retinal microglia after 4 weeks of STZ-induced diabetes. At this early time point in diabetes induction, single retinal cell suspensions were sorted via FACS using a Cd11b-FITC conjugate. RNA was isolated and preamplified using the SMARTSeq v4 low RNA input kit. After library preparation a 50bp single end read was performed at a depth of 19-34 million reads per sample using the Illumina HiSeq. Constructs were mapped to the rat genome and differential expression calculated between the STZ-treated and control groups. Overall design: Examination of 2 groups (control and diabetic) after 4 weeks of STZ induced diabetes
创建时间：
2021-12-17
