Expression data from left versus right mouse entorhinal cortex (EC)

The entorhinal cortex of the mouse seems to be sensitive to molecular mechanisms that have been linked to the pathology of Alzheimer's disease. In this microarray study we are interested in comparing the expression profile of the left versus the right EC of the mouse, in order to understand if there is a significant difference in gene expression that might reveal any insights into the differential activation of these areas. We used microarrays to detail the global programme of gene expression underlying a possible lateralization of the EC in the mouse brain (left versus right EC). The left and the right entorhinal cortices of 6 month-old C57BL/6 wild-type mice was dissected out by following anatomical landmarks and guided by the Mouse Brain Paxinos and Franklin's atlas. Samples were immediately processed for RNA extraction by using the RNeasy Kit from QIAGEN according to manufacturer's instructions. Before running the microarrays, RNA quality and integrity was monitored on an Agilent BioAnalyzer. Samples were then run on Affymetrix Genechip Mouse gene 2.0 ST arrays following Affymetrix's standard procedures (n=3 per cortical hemisphere). Microarrays data were analyzed through the use of Ingenuity iReport (Ingenuity Systems, www.ingenuity.com).小鼠的内嗅皮层（entorhinal cortex, EC）似乎对与阿尔茨海默病（Alzheimer's disease）病理相关的分子机制具有敏感性。本项基因微阵列研究旨在比较小鼠左侧与右侧内嗅皮层的表达谱，以探究二者是否存在显著的基因表达差异，进而为解析这两个脑区的差异化激活机制提供研究线索。我们借助基因微阵列技术，详细刻画了小鼠脑内（左侧与右侧内嗅皮层）潜在的内嗅皮层偏侧化所关联的全局基因表达程序。 我们参照解剖学标志，并以《Paxinos与Franklin小鼠脑图谱》为指导，从6月龄C57BL/6野生型小鼠体内分离得到左侧与右侧内嗅皮层。样本分离后立即按照QIAGEN公司RNeasy试剂盒的说明书进行RNA提取操作。在开展基因微阵列实验前，我们使用安捷伦生物分析仪（Agilent BioAnalyzer）检测了RNA的质量与完整性。随后我们按照Affymetrix的标准操作流程，将样本置于Affymetrix小鼠基因2.0 ST芯片上进行杂交检测，每个皮层半球设置3个生物学重复（n=3）。我们通过Ingenuity iReport软件（Ingenuity Systems公司，网址：www.ingenuity.com）对基因微阵列数据进行分析。