Mapping chromatin accessibility in stable cell lines expressing Drosophila Hox proteins, Hox cofactors Extradenticle and Homothorax, and the pioneer factor Gcm [ATAC-seq]

We have used stable cell lines expressing selected Hox proteins, Hox cofactors and the pioneer factor Glial cells missing, singly or in combination, to investigate the effects on chromatin accessibility genome-wide using ATAC-Seq. Overall design: We have examined chromatin accessibility in stable cell lines expressing selected Drosophila Hox proteins in the absence and presence of the cofactor Homothorax, in the presence of the pioneer factor Glial cells missing and in the presence of both Homothorax and Glial cells missing. We also examine chromatin accessibility in stable cell lines expressing either Homothorax or Glial cells missing. For each sample type there are two biological replicates for both induced (i) and non-induced (n) samples. For the analysis of chromatin accessibility in the basal Kc167 cells there are three biological replicates.