Ferroptosis-related Gene Signature Predicts Glioma Cell Death and Glioma Patient Progression

cell culture:The human glioma cell line U87MG was obtained from the Cell Resource Center, Peking Union Medical College (Beijing, China), and U251MG was acquired from the American Type Culture Collection (Manassas, VA). Temozolomide (TMZ) resistant U87MG cells (U87TR) and TMZ resistant U251MG cells (U251TR) of glioblastoma (GBM) sub-cell lines, were established through repetitive exposure to increasing TMZ concentrations in vitro in our laboratory. Cells were cultured in DMEM culture medium supplemented with 10% FBS with a standard humidified incubator under 5% CO2 at 37°C. Overall design: RNA sequencing of glioma cell lines:Total RNA from U251MG, U87MG, U251TR and U87TR cell lines were extracted and quantified. A total amount of 3 µg RNA per sample was used as input material for library preparation. The library preparations went through standard Illumina Hiseq platform and 150 bp paired-end reads from transcriptome sequencing were generated. After quality control procedures, the data were mapped to human hg38 reference by STAR (Dobin et al., 2013).