Genome-wide maps of chromatin accessibility in DN3 thymocytes and splenic naïve CD4 T cells.

We found that naïve T cells from SCD1-/- mice have an intrinsic preference for regulatory T cells differentiation. Such preference was imprinted to DN3 thymocytes developed in SCD1 deficient thymus and persisted to mature T cells. To investigate the potential epigenetic changes underlying the Treg-bias. We employed ATAC-seq to examine the chromatin accessibility of naïve T cells and DN3 thymocytes from SCD1-/- mice and their littermate controls. We purified spleen-derived naïve CD4 T cells and thymus-derived DN3 cells from both SCD1 knockout mice and their littermate controls. Harvested cells were subjected to examine chromatin accessibility using ATAC-seq.