Reproducibility Package for: Retrospective analysis of antimicrobial susceptibility testing illustrates the problem of resistant Staphylococcus species in cats

<p><strong>PI-Provided Abstract</strong>: Staphylococcus infections are a concern in both human and veterinary medicine due to their zoonotic potential, ability to cause a spectrum of diseases, and antimicrobial resistance (AMR). We retrospectively analyzed AMR among 1,458 Staphylococcus isolates obtained from cats over 15 years. Staphylococcus felis (24%), Staphylococcus aureus (21%), and Staphylococcus pseudintermedius (14%) were the prominent species. The most common isolation sites were skin and soft tissues (63%) and urinary tract (25%). Resistance to at least one antimicrobial was seen in 54% of isolates. S. epidermidis and S. pseudintermedius displayed the lowest proportion of susceptibility. Penicillin was the antimicrobial with the lowest overall susceptibility (55%), followed by erythromycin (61%) and ampicillin (64%), while vancomycin and chloramphenicol showed susceptibilities above 95%. The overall MDR proportion was 17%, and it was most common among S. pseudintermedius isolates (45%). Survival models revealed increased median minimum inhibitory concentration (MIC50) for ampicillin and cefovecin in 2018–2022 compared to 2008–2012 and decreasing doxycycline MIC50. Compared to other staphylococcal species, S. aureus had higher median MICs for most β-lactams, and S. pseudintermedius had higher MIC50 for non β-lactams. This study underscores the significance of AMR and MDR in feline medicine and One Health. The findings emphasize the need for vigilant AMR surveillance in veterinary practices and its association with antimicrobial prescription and use in companion animal medicine.</p>