The whole transcriptome regulation as a function of mitochondrial polymorphisms and age in C. elegans

The complex IV polymorphisms in mitochondria not only affected the mitochondria mass but also miRNA, lncRNA, mRNA and circRNA profiles. Furthermore, Most of genes that associated with mitochondria, showed age-related expression feature (P = 3.62156E-37). Next, we constructed a differentially expressed circRNA-lncRNA-miRNA-mRNA-ceRNA regulatory networks to determine functional interactions affected by the mitochondria variation. We also selected top 10 candidates ceRNA with the most correlation among the RNA. In addition, KEGG pathway analyses showed that the genes affected by the mitochondria variation were an enrichment of metabolic activity. Finally, we examined the metabolic changes in the old worms and analyzed the association between the metabolites and genes. The construction of multi-omics regulation network is great value for the research of nuclear genes mitochondria and aging. Here, we constructed a multi-level regulatory network with aging which affected by the mitochondria variation in C. elegans and the interaction between these genes are great value for further aging research. Thus, our finding provides new evidence for understanding the molecular mechanisms of how mitochondria influence on aging. lncRNA and mRNA profiles of 0-day, 6-day, 9-day, 12-day old N2 strain and the transnuclear CN30 worms were generated by deep sequencing, using Illumina HiSeq Xten platform.