Effects of temperature on metabolic rate during metamorphosis in the alfalfa leafcutting bee

Spring conditions, especially in temperate regions, may fluctuate abruptly and drastically. Environmental variability can expose organisms to temperatures outside of their optimal thermal ranges. For ectotherms, sudden changes in temperature may cause short- and long-term physiological effects, including changes in respiration, morphology, and reproduction. Exposure to variable temperatures during active development, which is likely to occur for insects developing in spring, can cause detrimental effects. Using the alfalfa leafcutting bee, Megachile rotundata, we aimed to determine if oxygen consumption could be measured using a new system and to test the hypothesis that female and male M. rotundata have a thermal performance curve with a wide optimal range. Oxygen consumption of M. rotundata pupae was measured across a large range of temperatures (6-48°C) using an optical oxygen sensor in a closed respirometry system. Absolute and mass-specific metabolic rates were calculated and compa..., , , # Effects of temperature on metabolic rate during metamorphosis in the alfafla leafcutting bee

* **Data for Microrez_Complete**

Column-Entry-Value-Unit-Explanation -Remark

A-Well_ID-A1-A6- B1-B6- C1-C6- D1-D6-The well number of the tested bee

B-SDR-757- 760--Microplate number

C-StartDate-Numerical--Date of collection

D-Temperature-Numerical-Degrees celsius-Temperature treatment/temperature recorded at

E-Treatment-Brood cell- extracted--Bees left in brood cells or bees extracted from brood cells

F-Sex-M- F- Unknown--Sex of bee tested determined at adult stage-NA value are bees that never made it to the adult stage and could not be sexed

G-Insect_Wt_g-Numerical-grams-Weight of bee tested

H-Bee_Volume-Numerical-ul-volume of bee tested 

I-Brood_cell_volume-Numerical-ul-Volume of brood cell without bee

J-Total_Weight-Numerical-grams-Weight of the bee and brood cell

K-Brood_Cell_Weight-Numerical-grams-Weight of brood cell without bee

L-Slope_PerAirSat_Hr-Numerical-% air sat...