ApoD modulation mouse cerebellar transcriptome

The lipocalin Apolipoprotein D (ApoD), known to protect the nervous system against oxidative stress (OS) in model organisms, is up-regulated early in the mouse brain in response to the ROS generator paraquat (PQ). However, the processes triggered by this up-regulation have not been explored. We present here a study of the effect of ApoD on the early transcriptional changes upon OS in the mouse cerebellum using microarray profiling. ApoD-KO and transgenic mice over-expressing ApoD in neurons are compared to wild-type controls.脂钙蛋白载脂蛋白D（lipocalin Apolipoprotein D, ApoD）已被证实可在模式生物中保护神经系统抵御氧化应激（oxidative stress, OS）。当活性氧发生器百草枯（paraquat, PQ）作用于小鼠脑部时，ApoD的表达会在早期被上调，但该上调事件所触发的生物学过程尚未得到探索。本研究采用基因芯片分析（microarray profiling）技术，探究了ApoD对小鼠小脑氧化应激下早期转录变化的影响，实验中将ApoD基因敲除（ApoD-KO）小鼠、神经元过表达ApoD的转基因小鼠与野生型对照小鼠进行了对比分析。