RNA-sequencing data for 923 enhancer deletion newborn mouse whole skin

We examined the RNA expression profiles of whole skin from newborn CRISPR-edited mice that have the 923 enhancer located within the Epidermial Differentiation Complex deleted. We examined two independent deletion lines, 923del and 923large, both homozygous mutants and heterozygotes, as well as wild type mice and involucrin Ivl knockout mice. We find that Ivl, the gene most proximal to 923, is downregulated upon the loss of the enhancer in both independent deletions, and 4 additional genes are also downregulated in both deletion lines, a secondary effect of the loss of Ivl, as concluded by comparing to the Ivl KO mice. We thus conclude that Ivl is the primary target gene of the 923 enhancer and establish a 923-Ivl regulatory module. Examination of RNA expression profiles in two 923 enhancer deletion lines, the Ivl knockout line and WT newborn mouse whole skins.本研究对CRISPR编辑的新生小鼠全皮肤的RNA表达谱进行了检测，该类小鼠的表皮分化复合物（Epidermial Differentiation Complex）内的923增强子（enhancer）已被定向删除。我们共纳入两个独立的缺失品系：923del与923large，涵盖其纯合突变体与杂合子个体，同时设置野生型小鼠及兜甲蛋白Ivl基因敲除（knockout, KO）小鼠作为对照群体。研究结果显示，距离923位点最近的基因Ivl在两个独立的增强子缺失品系中均出现表达下调；另有4个基因在两个缺失品系中同样呈现下调趋势，通过与Ivl KO小鼠的转录组数据对比可证实，该现象为Ivl缺失所引发的次级效应。据此我们推断，Ivl是923增强子的核心靶基因，并由此确立了923-Ivl调控模块。本研究的RNA表达谱检测样本涵盖上述两个923增强子缺失品系、Ivl基因敲除品系与野生型新生小鼠的全皮肤组织。